Applications in diseases processes
I. Elucidating the decoding mechanisms of ubiquitin signals and their significance in neurodegenerative/neurodevelopment diseases (as part of the Sagol School of Neuroscience)
Projects in the lab include: Molecular mechanisms that regulate membrane protein trafficking and Parkinson’s disease Mechanisms for regulation of sodium / potassium channels and dopamine transporter.
The molecular basis of the involvement of the ubiquitin E3-ligases in Autism and Kufmann syndromes
The involvement of ubiquitin-receptors in amyloid formation.
We developed E. coli models for these diseases in which the bacteria became addicted to functional expression of ubiquitylation cascades involve in the disease. A full description of the system is described in our Nature Methods paper
II. Pathogenic bacteria such as enterohaemorrhagic E. coli (EHEC) employ multiple strategies to evade, subvert and hijack the host cellular pathways for their own benefits. As the Ub system functions as a pivotal regulator, many of the effectors delivered by these pathogens target the Ub-system.
By employing the novel genetic system recently developed in our lab (Nature Methods) we identified and characterized several new E3-ligases from EHEC.
EHEC utilize Type-III secretion system to deliver protein effectors into the host. These effectors subvert the host cells and in many cases lead to death.