Selected Articals
Deubiquitylating enzymes in neuronal health and disease.
Amer-Sarsour F, Kordonsky A, Berdichevsky Y, Prag G, Ashkenazi A.
Cell Death Dis. 2021 Jan 22;12(1):120. doi: 10.1038/s41419-020-03361-5.
HK022 bacteriophage Integrase mediated RMCE as a potential tool for human gene therapy
Elias A, Kassis H, Elkader SA, Gritsenko N, Nahmad A, Shir H, Younis L, Shannan A, Aihara H, Prag G, Yagil E, Kolot M
Nucleic Acids Res. 2020 Dec 3:gkaa1140. doi: 10.1093/nar/gkaa1140.
An Integrative Synthetic Biology Approach to Interrogating Cellular Ubiquitin and Ufm Signaling
C Li, T Han, R Guo, P Chen, C Peng, G Prag and H Ronggui
IInt. J. Mol. Sci. 21, 4231; doi:10.3390/ijms21124231 2020
Remodeling Membrane Binding by Mono-Ubiquitylation
N Tanner, O Kleifeld, I Nachman, G Prag
Biomolecules 9 (8), 325 2019
Structural Insight into Regulation of the Proteasome Ub-Receptor Rpn10
T Keren-Kaplan, I Attali, O Levin-Kravets, O Kleifeld, S Ben-Aroya, G Prag
Ubiquitin Proteasome System-Current Insights into Mechanism Cellular …
2019
Regulation of the anaphase promoting complex/cyclosome by the degradation of its unassembled catalytic subunit, Apc11
M Volpe, N Levinton, N Rosenstein, G Prag, S Ben-Aroya
The FASEB Journal, fj. 201802300R 2019
Ubiquitin Signaling and Degradation of Aggregate-Prone Proteins
M Galves, R Rathi, G Prag, A Ashkenazi
Trends in biochemical sciences 2019
HK022 Bacteriophage Integrase: An Efficient Tool for Human Genome Manipulation and Therapy
A Elias, N Gritsenko, H Kassis, S Abd Alkader, A Barzel, E Yagil, G Prag, ...
MOLECULAR THERAPY 27 (4), 352-353 2019
A mutagenesis analysis of Tim50, the major receptor of the TIM23 complex, identifies regions that affect its interaction with Tim23
D Dayan, M Bandel, U Günsel, I Nussbaum, G Prag, D Mokranjac, ...
Scientific reports 9 (1), 2012 2019
Anti-cancer binary system activated by bacteriophage HK022 integrase
A Elias, N Gritsenko, R Gorovits, I Spector, G Prag, E Yagil, M Kolot
Oncotarget 9 (44), 27487 2018
E. coli-Based Selection and Expression Systems for Discovery, Characterization, and Purification of Ubiquitylated Proteins
O Levin-Kravets, T Keren-Kaplan, I Attali, I Sharon, N Tanner, D Shapira, ...
The Ubiquitin Proteasome System, 155-166 2018
Regulation mechanisms for writing, editing and reading the ubiquitin code
G Prag, O Levin-Kravets
FEBS JOURNAL 284, 43-43 2017
Novel anti-cancer binary system activated by bacteriophage HK022 Integrase
A Elias, I Spector, N Gritsenko, Y Zilberstein, R Gorovits, G Prag, M Kolot
FEBS JOURNAL 284, 258-258 2017
Ubiquitylation‐dependent oligomerization regulates activity of Nedd4 ligases
I Attali, WS Tobelaim, A Persaud, K Motamedchaboki, KJ Simpson‐Lavy, ...
The EMBO journal 36 (4), 425-440 2017
A bacterial genetic selection system for ubiquitylation cascade discovery
O Levin-Kravets, N Tanner, N Shohat, I Attali, T Keren-Kaplan, ...
Nature methods 13 (11), 945 2016
Structure of ubiquitylated-Rpn10 provides insight into its autoregulation mechanism
T Keren-Kaplan, LZ Peters, O Levin-Kravets, I Attali, O Kleifeld, N Shohat, ...
Nature communications 7, 12960 2016
Tetrameric assembly of monoubiquitin accurately mimics the Lys11 polyubiquitin chain structure
O Levin-Kravets, N Shohat, G Prag
Biochemistry 54 (30), 4704-4710 2015
A multilaboratory comparison of calibration accuracy and the performance of external references in analytical ultracentrifugation
H Zhao, R Ghirlando, C Alfonso, F Arisaka, I Attali, DL Bain, MM Bakhtina, ...
PLoS One 10 (5), e0126420 2015
Structure‐based in silico identification of ubiquitin‐binding domains provides insights into the ALIX‐V: ubiquitin complex and retrovirus budding
T Keren‐Kaplan, I Attali, M Estrin, LS Kuo, E Farkash, ...
The EMBO journal 32 (4), 538-551 2013
Purification and crystallization of mono-ubiquitylated ubiquitin receptor Rpn10
T Keren-Kaplan, G Prag
Acta Crystallographica Section F: Structural Biology and Crystallization … 2012
Purification and crystallization of yeast Ent1 ENTH domain
N Tanner, G Prag
Acta Crystallographica Section F: Structural Biology and Crystallization …2012
The hetero-hexameric nature of a chloroplast AAA+ FtsH protease contributes to its thermodynamic stability
O Moldavski, O Levin-Kravets, T Ziv, Z Adam, G Prag
PLoS One 7 (4), e36008 2012
Synthetic biology approach to reconstituting the ubiquitylation cascade in bacteria
T Keren‐Kaplan, I Attali, K Motamedchaboki, BA Davis, N Tanner, ...
The EMBO journal 31 (2), 378-390 2012
A phage display system designed to detect and study protein–protein interactions
CL Bair, A Oppenheim, A Trostel, G Prag, S Adhya
Molecular microbiology 67 (4), 719-728 2008
The Vps27/Hse1 complex is a GAT domain-based scaffold for ubiquitin-dependent sorting
G Prag, H Watson, YC Kim, BM Beach, R Ghirlando, G Hummer, ...
Developmental cell 12 (6), 973-986 2007
Ubiquitin-binding domains
JH Hurley, S Lee, G Prag
Biochemical Journal 399 (3), 361-372 2006
Structural mechanism for ubiquitinated-cargo recognition by the Golgi-localized,
γ-ear-containing, ADP-ribosylation-factor-binding proteins
G Prag, S Lee, R Mattera, CN Arighi, BM Beach, JS Bonifacino, JH Hurley
Proceedings of the National Academy of Sciences 102 (7), 2334-2339 2005
Structure of the ESCRT-II endosomal trafficking complex
A Hierro, J Sun, AS Rusnak, J Kim, G Prag, SD Emr, JH Hurley
Nature 431 (7005), 221 2004
Mechanism of ubiquitin recognition by the CUE domain of Vps9p
G Prag, S Misra, EA Jones, R Ghirlando, BA Davies, BF Horazdovsky, ...
Cell 113 (5), 609-620 2003
A ubiquitin‐binding motif required for intramolecular monoubiquitylation, the CUE domain
SC Shih, G Prag, SA Francis, MA Sutanto, JH Hurley, L Hicke
The EMBO journal 22 (6), 1273-1281 2003
Crystal structure of a new ubiquitin binding domain, the CUE domain of yeast Vps9p
S Misra, G Prag, E Jones, JH Hurley
BIOPHYSICAL JOURNAL 84 (2), 62A-62A 2003
High Resolution Structural Analyses of Mutant Chitinase A Complexes with Substrates Provide New Insight into the Mechanism of Catalysis,
Y Papanikolau, G Prag, G Tavlas, CE Vorgias, AB Oppenheim, K Petratos
Biochemistry 40 (38), 11338-11343 2001
Structures of chitobiase mutants complexed with the substrate di-N-acetyl-D-glucosamine: the catalytic role of the conserved acidic pair, aspartate 539 and glutamate 540
G Prag, Y Papanikolau, G Tavlas, CE Vorgias, K Petratos, AB Oppenheim
Journal of molecular biology 300 (3), 611-617 2000
Structural studies of chitinase A from S. marcescens complexed with substrates and the inhibitor
Y Papanikolau, G Prag, G Tavlas, M Papadovasilaki, S Dimitriou, ...
ACTA CRYSTALLOGRAPHICA A-FOUNDATION AND ADVANCES 56, S259-S259 2000
Trapping intermediate structures of chitin degrading enzymes with native substrate
G Prag, A Oppenheim, PA Tucker
EMBL Annual Reports 2000