Selected Articals

Deubiquitylating enzymes in neuronal health and disease.

Amer-Sarsour F, Kordonsky A, Berdichevsky Y, Prag G, Ashkenazi A.

Cell Death Dis. 2021 Jan 22;12(1):120. doi: 10.1038/s41419-020-03361-5.

HK022 bacteriophage Integrase mediated RMCE as a potential tool for human gene therapy
Elias A, Kassis H, Elkader SA, Gritsenko N, Nahmad A, Shir H, Younis L, Shannan A, Aihara H, Prag G, Yagil E, Kolot M
Nucleic Acids Res. 2020 Dec 3:gkaa1140. doi: 10.1093/nar/gkaa1140.
An Integrative Synthetic Biology Approach to Interrogating Cellular Ubiquitin and Ufm Signaling ‏
C Li, T Han, R Guo, P Chen, C Peng, G Prag and H Ronggui ‏
IInt. J. Mol. Sci.  21, 4231; doi:10.3390/ijms21124231 2020
Remodeling Membrane Binding by Mono-Ubiquitylation‏
N Tanner, O Kleifeld, I Nachman, G Prag‏
Biomolecules 9 (8), 325 ‏       2019
Structural Insight into Regulation of the Proteasome Ub-Receptor Rpn10‏
T Keren-Kaplan, I Attali, O Levin-Kravets, O Kleifeld, S Ben-Aroya, G Prag‏
Ubiquitin Proteasome System-Current Insights into Mechanism Cellular …‏
Regulation of the anaphase promoting complex/cyclosome by the degradation of its unassembled catalytic subunit, Apc11‏
M Volpe, N Levinton, N Rosenstein, G Prag, S Ben-Aroya‏
The FASEB Journal, fj. 201802300R 2019
Ubiquitin Signaling and Degradation of Aggregate-Prone Proteins‏
M Galves, R Rathi, G Prag, A Ashkenazi‏
Trends in biochemical sciences ‏         2019
HK022 Bacteriophage Integrase: An Efficient Tool for Human Genome Manipulation and Therapy‏
A Elias, N Gritsenko, H Kassis, S Abd Alkader, A Barzel, E Yagil, G Prag, ...‏
MOLECULAR THERAPY 27 (4), 352-353 2019   
A mutagenesis analysis of Tim50, the major receptor of the TIM23 complex, identifies regions that affect its interaction with Tim23‏
D Dayan, M Bandel, U Günsel, I Nussbaum, G Prag, D Mokranjac, ...‏
Scientific reports 9 (1), 2012 2019
Anti-cancer binary system activated by bacteriophage HK022 integrase‏
A Elias, N Gritsenko, R Gorovits, I Spector, G Prag, E Yagil, M Kolot‏
Oncotarget 9 (44), 27487 2018
E. coli-Based Selection and Expression Systems for Discovery, Characterization, and Purification of Ubiquitylated Proteins‏
O Levin-Kravets, T Keren-Kaplan, I Attali, I Sharon, N Tanner, D Shapira, ...‏
The Ubiquitin Proteasome System, 155-166 2018
Regulation mechanisms for writing, editing and reading the ubiquitin code‏
G Prag, O Levin-Kravets‏
FEBS JOURNAL 284, 43-43 2017
Novel anti-cancer binary system activated by bacteriophage HK022 Integrase‏
A Elias, I Spector, N Gritsenko, Y Zilberstein, R Gorovits, G Prag, M Kolot‏
FEBS JOURNAL 284, 258-258 2017
Ubiquitylation‐dependent oligomerization regulates activity of Nedd4 ligases‏
I Attali, WS Tobelaim, A Persaud, K Motamedchaboki, KJ Simpson‐Lavy, ...‏
The EMBO journal 36 (4), 425-440 2017
A bacterial genetic selection system for ubiquitylation cascade discovery‏
O Levin-Kravets, N Tanner, N Shohat, I Attali, T Keren-Kaplan, ...‏
Nature methods 13 (11), 945 2016
Structure of ubiquitylated-Rpn10 provides insight into its autoregulation mechanism‏
T Keren-Kaplan, LZ Peters, O Levin-Kravets, I Attali, O Kleifeld, N Shohat, ...‏
Nature communications 7, 12960 2016
Tetrameric assembly of monoubiquitin accurately mimics the Lys11 polyubiquitin chain structure‏
O Levin-Kravets, N Shohat, G Prag‏
Biochemistry 54 (30), 4704-4710 2015
A multilaboratory comparison of calibration accuracy and the performance of external references in analytical ultracentrifugation‏
H Zhao, R Ghirlando, C Alfonso, F Arisaka, I Attali, DL Bain, MM Bakhtina, ...‏
PLoS One 10 (5), e0126420 2015
Structure‐based in silico identification of ubiquitin‐binding domains provides insights into the ALIX‐V: ubiquitin complex and retrovirus budding‏
T Keren‐Kaplan, I Attali, M Estrin, LS Kuo, E Farkash, ...‏
The EMBO journal 32 (4), 538-551 2013
Purification and crystallization of mono-ubiquitylated ubiquitin receptor Rpn10‏
T Keren-Kaplan, G Prag‏
Acta Crystallographica Section F: Structural Biology and Crystallization …‏  2012
Purification and crystallization of yeast Ent1 ENTH domain‏
N Tanner, G Prag‏
Acta Crystallographica Section F: Structural Biology and Crystallization …2012
The hetero-hexameric nature of a chloroplast AAA+ FtsH protease contributes to its thermodynamic stability‏
O Moldavski, O Levin-Kravets, T Ziv, Z Adam, G Prag‏

PLoS One 7 (4), e36008 2012


Synthetic biology approach to reconstituting the ubiquitylation cascade in bacteria‏

T Keren‐Kaplan, I Attali, K Motamedchaboki, BA Davis, N Tanner, ...‏
The EMBO journal 31 (2), 378-390 2012
A phage display system designed to detect and study protein–protein interactions‏
CL Bair, A Oppenheim, A Trostel, G Prag, S Adhya‏
Molecular microbiology 67 (4), 719-728 2008
The Vps27/Hse1 complex is a GAT domain-based scaffold for ubiquitin-dependent sorting‏
G Prag, H Watson, YC Kim, BM Beach, R Ghirlando, G Hummer, ...‏
Developmental cell 12 (6), 973-986 2007
Ubiquitin-binding domains‏
JH Hurley, S Lee, G Prag‏
Biochemical Journal 399 (3), 361-372 2006
Structural mechanism for ubiquitinated-cargo recognition by the Golgi-localized,
γ-ear-containing, ADP-ribosylation-factor-binding proteins‏
G Prag, S Lee, R Mattera, CN Arighi, BM Beach, JS Bonifacino, JH Hurley‏
Proceedings of the National Academy of Sciences 102 (7), 2334-2339 2005
Structure of the ESCRT-II endosomal trafficking complex‏
A Hierro, J Sun, AS Rusnak, J Kim, G Prag, SD Emr, JH Hurley‏
Nature 431 (7005), 221 2004
Mechanism of ubiquitin recognition by the CUE domain of Vps9p‏
G Prag, S Misra, EA Jones, R Ghirlando, BA Davies, BF Horazdovsky, ...‏
Cell 113 (5), 609-620 2003
A ubiquitin‐binding motif required for intramolecular monoubiquitylation, the CUE domain‏
SC Shih, G Prag, SA Francis, MA Sutanto, JH Hurley, L Hicke‏
The EMBO journal 22 (6), 1273-1281 2003
Crystal structure of a new ubiquitin binding domain, the CUE domain of yeast Vps9p‏
S Misra, G Prag, E Jones, JH Hurley‏
BIOPHYSICAL JOURNAL 84 (2), 62A-62A 2003
High Resolution Structural Analyses of Mutant Chitinase A Complexes with Substrates Provide New Insight into the Mechanism of Catalysis,‏
Y Papanikolau, G Prag, G Tavlas, CE Vorgias, AB Oppenheim, K Petratos‏
Biochemistry 40 (38), 11338-11343 2001
Structures of chitobiase mutants complexed with the substrate di-N-acetyl-D-glucosamine: the catalytic role of the conserved acidic pair, aspartate 539 and glutamate 540‏
G Prag, Y Papanikolau, G Tavlas, CE Vorgias, K Petratos, AB Oppenheim‏
Journal of molecular biology 300 (3), 611-617 2000
Structural studies of chitinase A from S. marcescens complexed with substrates and the inhibitor‏
Y Papanikolau, G Prag, G Tavlas, M Papadovasilaki, S Dimitriou, ...‏
Trapping intermediate structures of chitin degrading enzymes with native substrate‏
G Prag, A Oppenheim, PA Tucker 

EMBL Annual Reports 2000

Participation of IHF and a distant UP element in the stimulation of the phage λ PL promoter‏
H Giladi, S Koby, G Prag, M Engelhorn, J Geiselmann, AB Oppenheim‏
Molecular microbiology 30 (2), 443-451 1998
Structural principles of prokaryotic gene regulatory proteins and the evolution of repressors and gene activators‏
G Prag, S Greenberg, AB Oppenheim‏
Molecular microbiology 26 (3), 619-620 1997