Selected Articals
E. coli-Based Selection and Expression Systems for Discovery, Characterization, and Purification of Ubiquitylated Proteins
Levin-Kravets O, Keren-Kaplan T, Attali I, Sharon I, Tanner N, Shapira D, Rathi R, Persaud A, Shohat N, Shusterman A, Prag G.
Methods Mol Biol. 2018;1844:155-166. doi: 10.1007/978-1-4939-8706-1_11
Anti-cancer binary system activated by bacteriophage HK022 integrase
Elias A, Gritsenko N, Gorovits R, Spector I, Prag G, Yagil E, Kolot M.
Oncotarget. 2018 Jun 8;9(44):27487-27501
Ubiquitylation-dependent oligomerization regulates activity of Nedd4 ligases.
Attali I, Tobelaim WS, Persaud A, Motamedchaboki K, Simpson-Lavy KJ, Mashahreh B, Levin-Kravets O, Keren-Kaplan T, Pilzer I, Kupiec M, Wiener R, Wolf DA, Rotin D, Prag G.
EMBO J. 2017 Jan 9. pii: e201694314. doi: 10.15252/embj.201694314.
* See a highlighted in the EMBO J. News & Views
Structure of ubiquitylated-Rpn10 provides insight into its autoregulation mechanism.
Keren-Kaplan T, Zeev Peters L, Levin-Kravets O, Attali I, Kleifeld O, Shohat N, Artzi S, Zucker O, Pilzer I, Reis N, Glickman MH, Ben-Aroya S, Prag G.
Nature Communications. 2016 Oct 4;7:12960. doi: 10.1038/ncomms12960.
A bacterial genetic selection system for ubiquitylation cascade discovery.
Levin-Kravets O, Tanner N, Shohat N, Attali I, Keren-Kaplan T, Shusterman A, Artzi S, Varvak A, Reshef Y, Shi X, Zucker O, Baram T, Katina C, Pilzer I, Ben-Aroya S, Prag G.
Nature Methods. 2016 Oct 3. doi: 10.1038/nmeth.4003.
Tetrameric Assembly of Monoubiquitin Accurately Mimics the Lys11 Polyubiquitin Chain Structure.
Levin-Kravets O, Shohat N, Prag G.
Biochemistry. 2015 Jul 22. [Epub ahead of print]
A multilaboratory comparison of calibration accuracy and the performance of external references in analytical ultracentrifugation.
Zhao H, et al.
PLoS One. 2015 May 21;10(5):e0126420. doi: 10.1371/journal.pone.0126420. eCollection 2015.
Structure-based in silico identification of ubiquitin-binding domains provides insights into the ALIX-V:ubiquitin complex and retrovirus budding.
Keren-Kaplan T, Attali I, Estrin M, Kuo LS, Farkash E, Jerabek-Willemsen M, Blutraich N, Artzi S, Peri A, Freed EO, Wolfson HJ, Prag G.
EMBO J. 2013 Feb 20;32(4):538-51. doi: 10.1038/emboj.2013.4. Epub 2013 Jan 29.
Purification and crystallization of mono-ubiquitylated ubiquitin receptor Rpn10.
Keren-Kaplan T, Prag G.
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 Sep 1;68(Pt 9):1120-3. doi: 10.1107/S1744309112034331. Epub 2012 Aug 31.
Purification and crystallization of yeast Ent1 ENTH domain.
Tanner N, Prag G.
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 Jul 1;68(Pt 7):820-3. doi: 10.1107/S1744309112022488. Epub 2012 Jun 28.
The hetero-hexameric nature of a chloroplast AAA+ FtsH protease contributes to its thermodynamic stability.
Moldavski O, Levin-Kravets O, Ziv T, Adam Z, Prag G.
PLoS One. 2012;7(4):e36008. doi: 10.1371/journal.pone.0036008. Epub 2012 Apr 27.
Synthetic biology approach to reconstituting the ubiquitylation cascade in bacteria.
Keren-Kaplan T, Attali I, Motamedchaboki K, Davis BA, Tanner N, Reshef Y, Laudon E, Kolot M, Levin-Kravets O, Kleifeld O, Glickman M, Horazdovsky BF, Wolf DA, Prag G.
EMBO J. 2012 Jan 18;31(2):378-90. doi: 10.1038/emboj.2011.397. Epub 2011 Nov 11.
* See a highlight in Nature Methods
A phage display system designed to detect and study protein-protein interactions.
Bair CL, Oppenheim A, Trostel A, Prag G, Adhya S.
Mol Microbiol. 2008 Feb;67(4):719-28.
The Vps27/Hse1 complex is a GAT domain-based scaffold for ubiquitin-dependent sorting.
Prag G, Watson H, Kim YC, Beach BM, Ghirlando R, Hummer G, Bonifacino JS, Hurley JH.
Dev Cell. 2007 Jun;12(6):973-86.
Ubiquitin-binding domains.
Hurley JH, Lee S, Prag G.
Biochem J. 2006 Nov 1;399(3):361-72. Review.
Structural mechanism for ubiquitinated-cargo recognition by the Golgi-localized, gamma-ear-containing, ADP-ribosylation-factor-binding proteins.
Prag G, Lee S, Mattera R, Arighi CN, Beach BM, Bonifacino JS, Hurley JH.
Proc Natl Acad Sci U S A. 2005 Feb 15;102(7):2334-9.
Structure of the ESCRT-II endosomal trafficking complex.
Hierro A, Sun J, Rusnak AS, Kim J, Prag G, Emr SD, Hurley JH.
Nature. 2004 Sep 9;431(7005):221-5. Epub 2004 Aug 25.
Mechanism of ubiquitin recognition by the CUE domain of Vps9p.
Prag G, Misra S, Jones EA, Ghirlando R, Davies BA, Horazdovsky BF, Hurley JH.
Cell. 2003 May 30;113(5):609-20.
A ubiquitin-binding motif required for intramolecular monoubiquitylation, the CUE domain.
Shih SC, Prag G, Francis SA, Sutanto MA, Hurley JH, Hicke L.
EMBO J. 2003 Mar 17;22(6):1273-81.
High resolution structural analyses of mutant chitinase A complexes with substrates provide new insight into the mechanism of catalysis.
Papanikolau Y, Prag G, Tavlas G, Vorgias CE, Oppenheim AB, Petratos K.
Biochemistry. 2001 Sep 25;40(38):11338-43.
Conservation of structural elements and catalytic mechanism in the chitinolytic enzymes from Serratia marcescens
Prag G, Vorgias CE, Oppenheim BA.
Chitin Enzymology, 2001, 3, 351-60
Structural studies of chitinase A from S. marcescens complexed with substrates and the inhibitor allosamidin
Y. Papanikolau, G. Prag, G. Tavlas, M. Papadovasilaki, S. Dimitriou,C. E. Vorgias, A. B. Oppenheim, K. Petratos
Acta Crystallogr Section A, 2000, 8
Structures of chitobiase mutants complexed with the substrate Di-N-acetyl-d-glucosamine: the catalytic role of the conserved acidic pair, aspartate 539 and glutamate 540.
Prag G, Papanikolau Y, Tavlas G, Vorgias CE, Petratos K, Oppenheim AB.
J Mol Biol. 2000 Jul 14;300(3):611-7.
Participation of IHF and a distant UP element in the stimulation of the phage lambda PL promoter.
Giladi H, Koby S, Prag G, Engelhorn M, Geiselmann J, Oppenheim AB.
Mol Microbiol. 1998 Oct;30(2):443-51.
Structural principles of prokaryotic gene regulatory proteins and the evolution of repressors and gene activators.
Prag G, Greenberg S, Oppenheim AB.
Mol Microbiol. 1997 Nov;26(3):619-20.